On the Artificial Pollen Grain Germination of Zea Mays L
نویسندگان
چکیده
منابع مشابه
Maize (Zea mays L.).
Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation proced...
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متن کاملGermination and seedling growth of corn (Zea mays l.) under varying levels of copper and zinc
The heavy metal tolerance in corn (Zea mays L.) var. ‘Neelum’ was assessed at germination and seedling growth after having subjected it to different concentrations of CuSO4 and ZnSO4. Germination was not affected by any of the metal tested, whereas initial growth was strongly inhibited by increasing concentrations of ZnSO4. Seedlings developed toxicity symptoms in the presence of both metals bu...
متن کاملFlow Cytometric Characteristics of Sperm Cells Isolated from Pollen of Zea mays L.
Sperm cells have been isolated from pollen of maize (Zea mays L.) and purified with Percoll density centrifugation. Their flow cytometric characteristics were determined on a FACScan flow cytometer with the fluorescent dyes, fluorescein diacetate and propidium iodide. Freshly isolated sperm cells appeared as a dot cluster on the forward scatter and side scatter dot plot. This dot cluster contai...
متن کاملSome physical properties of teosinte (Zea mays subsp. parviglumis) pollen.
In parts of the world where teosinte and maize are grown in close proximity, there is concern about gene flow between them. Pollen is the primary vehicle for gene flow. Quantifying the biophysical properties of pollen, such as its settling speed and dehydration rate, is important for evaluating outcrossing potential. These properties were measured for teosinte (Zea mays subsp. parviglumis) poll...
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ژورنال
عنوان ژورنال: Shokubutsugaku Zasshi
سال: 1958
ISSN: 0006-808X
DOI: 10.15281/jplantres1887.71.282